1. Thirteen adult females and two males were overfed a total of 79-1 59 MJ (1900G38 000 kcal) during a 3-week
period at the Clinical Research Center, Rochester. The average energy cost of the weight gain was 28 kJ
(6.7 kcal)/g, and about half the gain consisted of lean body mass (LBM) as estimated by 40K counting.
2. A survey of the literature disclosed twenty-eight normal males and five females who had been overfed a total
of 104362 MJ (2500&87000 kcal) under controlled conditions: twenty-five of these had assays of body
composition, and three had complete nitrogen balances.
3. When these values were combined with those from our subjects (total forty-eight), there was a significant
correlation between weight gain and total excess energy consumed (r 0.77, P < 0.01) and between LBM gain and
excess energy (r 0.49, P < 0.01). Based on means the energy cost was 33.7 kJ (8.05 kcal)/g gain and 43.6% of
the gain was LBM; from regression analysis these values were 33.7 kJ (8.05 kcal)/g gain and 38.4% of gain as
4. Indiv dual variations in the response could not be explained on the basis of sex, initial body-weight or fat content, duration of overfeeding, type of food eaten, amount of daily food consumption or, in a subset of subjects,
on smoking behaviour.
5. The average energy cost of the weight gain was close to the theoretical value of 33.8 kJ (8.08 kcal)/g derived
from the composition of the tissue gained.